Michael G Klein
EducationBS University of Massachusetts, Amherst MA
MS, PhD University of Illinois, Urbana-Champaign IL
BiographyI am interested in cellular physiology related to signaling mechanisms in heart cells.
Our ongoing research interests involve investigation of cardiac electrophysiology in three experimental model systems, i) isolated cardiac myocytes from large animals (swine), ii) human heart cells derived from induced pluripotent stem cells (hiPSC CMs) and iii) heterologous expression of ion channel proteins associated with electrical excitation in the heart. Three ongoing research projects involve i) characterization of cardiac toxicity of opioids, including methadone, loperamide and mitragynine, ii) regulation of Kir2 activity which is responsible for the inward rectifier current (IK1) and iii) medium-throughput electrical and optical measurements of heart cell functional activity.
Klein MG, Krantz MJ, Fatima N, et al. Methadone Blockade of Cardiac Inward Rectifier K+ Current Augments Membrane Instability and Amplifies U Waves on Surface ECGs: A Translational Study. J Am Heart Assoc. 2022;11(11):e023482. doi:10.1161/JAHA.121.023482
Geiger RM, Klein MG, Fatima N, Goldstein RE, Krantz,MJ Haigney MC, Flagg TP. Rapid Assessment of Proarrhythmic Potential Using Human iPSC-Derived Cardiomyocytes. JACC Clin Electrophysiol. 2020 Dec;6(14):1860-1862. doi: 10.1016/j.jacep.2020.08.034
Ostrominski JW, Yada RC, Sato N, Klein MG, Blinova K, Patel D, Valadez R, Palisoc M, Pittaluga S, Peng KW, San H, Lin Y, Basuli F, Zhang X, Swenson RE, Haigney M, Choyke PL, Zou J, Boehm M, Hong SG, Dunbar CE. CRISPR/Cas9-mediated introduction of the sodium/iodide symporter gene enables non-invasive in vivo tracking of iPSC-derived cardiomyocytes. STEM CELLS Transl Med. 2020;9:1203–1217.https://doi.org/10.1002/sctm.20-0019